maxquant-proteomics
MaxQuant + Perseus proteomics pipeline: configure and run MaxQuant for label-free quantification (LFQ) and SILAC; parse proteinGroups.txt in Python; filter contaminants/reverse decoys; log2-transform and median-normalize LFQ intensities; impute MNAR missing values; t-test with FDR correction; volcano plot; GO/pathway enrichment. Use Proteome Discoverer for Thermo instrument-native processing; FragPipe/MSFragger for GPU-accelerated database search.
Installation and usage
MaxQuant + Perseus proteomics pipeline: configure and run MaxQuant for label-free quantification (LFQ) and SILAC; parse proteinGroups.txt in Python; filter contaminants/reverse decoys; log2-transform and median-normalize LFQ intensities; impute MNAR missing values; t-test with FDR correction; volcano plot; GO/pathway enrichment. Use Proteome Discoverer for Thermo instrument-native processing; FragPipe/MSFragger for GPU-accelerated database search.
์ค์น ํ ํฐ๋ฏธ๋์์ ๋ค์ ๋ช ๋ น์ ์คํํ์ฌ ์ด ์คํฌ์ ์ฌ์ฉํ ์ ์์ต๋๋ค:
skills use maxquant-proteomics